methyldetector bisulfite modification kit (Active Motif)
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methyldetector bisulfite modification kit
Methyldetector Bisulfite Modification Kit, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/methyldetector+bisulfite+modification+kit/methyldetector+bisulfite+modification+kit/pmc11626423-99-12-16
Average 90 stars, based on 1 article reviews
Methyldetector Bisulfite Modification Kit, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/methyldetector+bisulfite+modification+kit/methyldetector+bisulfite+modification+kit/pmc11626423-99-12-16
Average 90 stars, based on 1 article reviews
methyldetector bisulfite modification kit - by Bioz Stars,
2026-09
90/100 stars
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1) Product Images from "Tissue factor signalling modifies the expression and regulation of G1/S checkpoint regulators: Implications during injury and prolonged inflammation"
Article Title: Tissue factor signalling modifies the expression and regulation of G1/S checkpoint regulators: Implications during injury and prolonged inflammation
Journal: Molecular Medicine Reports
doi: 10.3892/mmr.2024.13404
Figure Legend Snippet: Immortalised human epithelial cells (human telomerase reverse transcriptase-human pancreatic nestin-expressing ductal cells) were supplemented with recombinant TF (0.5 U/ml) or were untreated. The cells were harvested on week 4, gDNA was extracted from the cells (3×10 4 cells) and bisulphite conversion of the gDNA (750 ng) was carried out using the MethylDetector Bisulfite Modification Kit. DNA was also extracted from HT-29 cells (methylated control) and HDBEC (unmethylated control) and processed as aforementioned. The modified DNA (10 ng/reaction) was amplified with methylation-specific, and unmethylated-specific sets primers to the p16 gene promoter region. Each of the nested amplification steps was carried out for 35 cycles at an annealing temperature of 60°C. Aliquots (4 µl) from the outer reactions were then used as the template for the inner PCR reactions using primers specific for methylated and unmethylated DNA. Both amplicons generated bands of 149 bp. A control β-actin sample was also amplified and examined alongside. (A) The products were examined by 2% (w/v) agarose gel electrophoresis and (B) the band intensities determined and the ratios of the methylated:unmethylated DNA calculated (n=3). TF, tissue factor; gDNA, genomic DNA.
Techniques Used: Reverse Transcription, Expressing, Recombinant, Modification, Methylation, Control, Amplification, Generated, Agarose Gel Electrophoresis
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Modification:Article Title: Efficient Editing of the ZBED6-Binding Site in Intron 3 of IGF2 in a Bovine Model Using the CRISPR/Cas9 System Article Snippet: Genomic DNA was extracted from bovine ears following the protocol of the TIANamp Genomic DNA Kit (TIANGEN, Beijing, China). .. Approximately 100–500 ng of genomic DNA was subjected to bisulfite treatment using the Article Title: Tissue factor signalling modifies the expression and regulation of G1/S checkpoint regulators: Implications during injury and prolonged inflammation. Article Snippet: Genomic DNA (gDNA) was extracted from HPNE cells (3x104 cells) using the Monarch Genomic DNA purification kit (new england Biolabs, inc.) according to manufacturer's instructions. .. Bisulphite conversion of the gdna (750 ng) was carried out using the Article Title: Consistency analysis of microRNA‐arm expression reveals microRNA‐369‐5p/3p as tumor suppressors in gastric cancer Article Snippet: .. Bisulfite conversion was conducted using the Article Title: Tissue factor signalling modifies the expression and regulation of G1/S checkpoint regulators: Implications during injury and prolonged inflammation Article Snippet: Genomic DNA (gDNA) was extracted from HPNE cells (3×10 4 cells) using the Monarch Genomic DNA purification kit (New England BioLabs, Inc.) according to manufacturer's instructions. .. Bisulphite conversion of the gDNA (750 ng) was carried out using the Article Title: Methylation of promoter region of CDX2 gene in colorectal cancer Article Snippet: DNA extracted from the sample tissue was in accordance with the instructions of the animal cell genomic extraction kit, purchased from Axygen Scientific Inc. .. The modification of DNA bisulfite was performed in accordance with the instructions of the Article Title: Distinct Receptor Tyrosine Kinase Subsets Mediate Anti-HER2 Drug Resistance in Breast Cancer Article Snippet: .. Bisulfite conversion was performed using the Article Title: Age estimation by DNA methylation in the Antarctic minke whale Article Snippet: Determining the age of wild animals is very important in the study of their ecology and for stock assessment and management.. In baleen whales, the most common approach for age estimation is by counting the growth layers appearing in earplugs.. Although this method is actually considered the most reliable tool for age determination in whales, it cannot be performed on free-ranging individuals. Article Title: Molecular Cloning, Epigenetic Regulation and Functional Characterization of Prkd1 Gene Promoter in Dopaminergic Cell Culture Models of Parkinson’s Disease Article Snippet: .. Two micrograms of genomic DNA was bisulfite-modified and purified using Active Motif’s Purification:Article Title: Molecular Cloning, Epigenetic Regulation and Functional Characterization of Prkd1 Gene Promoter in Dopaminergic Cell Culture Models of Parkinson’s Disease Article Snippet: .. Two micrograms of genomic DNA was bisulfite-modified and purified using Active Motif’s |